Protein expression and modifications play key roles in regulating the cell’s function and human disease state. We mainly focuses on the protein expression changes in brain aging, neurodegenerative diseases, cancer cell surface protein targets and biomarkers by quantitative proteomics. Ongoing projects include protein quantification, acetylation and phosphorylation characterization in AD and PD, high throughput profiling of CRC cancer cell surface specific protein targets and development of the new antibody-drug conjugates.